Plasmid cloning

  • PCR
  • library construction
  • site-directed mutagenesis
  • automatic sequencing  

Bacterial protein expression systems

Protein purification and detection

  • electrophoresis, Western blotting, densitometry
  • UV/VIS- spectroscopy (cuvette and 96-well format)

Protein/Protein interaction

  • ToxR/POSSYCCAT system
  • Enzyme assays
  • ultracentrifugation
  • Liposome-Liposome fusion
  • fluorescence spectroscopy (cuvette and 96-well format)

Protein and peptide structure analysis

  • Circular dichroism spectroscopy
  • Hydrogen/Deuterium exchange
  • ESI-IMS-TOF tandem mass spectrometry (ESI-Q-TOF, MS or MS/MS with ETD and ion mobility mass spectrometry function (Synapt G2, Waters)
  • LC/MS ESI-TOF mass spectrometry (Agilent ToF)