Plasmid cloning
- PCR
- library construction
- site-directed mutagenesis
- automatic sequencing
Protein purification and detection
- electrophoresis, Western blotting, densitometry
- UV/VIS- spectroscopy (cuvette and 96-well format)
- HPLC, FPLC
Protein/Protein interaction
- ToxR/POSSYCCAT system
- Enzyme assays
- ultracentrifugation
- Liposome-Liposome fusion
- fluorescence spectroscopy (cuvette and 96-well format)
Protein and peptide structure analysis
- Circular dichroism spectroscopy
- Hydrogen/Deuterium exchange
- ESI-IMS-TOF tandem mass spectrometry (ESI-Q-TOF, MS or MS/MS with ETD and ion mobility mass spectrometry function (Synapt G2, Waters)
- LC/MS ESI-TOF mass spectrometry (Agilent ToF)
