Transmembrane helix / lipid interactions
Methods to investigate TMD / lipid interactions
We are currently working on a FRET-based assay to investigate the interaction between synthetic TMD peptides and fluorescently-labeled phospholipids. Further, we investigate deformation of lipid molecules that surround TMDs as a potential prelude to fusion.
In parallel, we perform Molecular dynamics simulations that can provide information on the different modes by which proteins and lipids can specifically recognize each others. The simulations also show how the lipid environment influences the backbone dynamics of a TMD.