PD. DR. KARL KRAMER

Position: Group Leader Cell Biology
Room: 005
Category: Scientific Staff
Phone: +49 - (0)8161 - 714477
Email: karl.kramer[at]tum.de

Publications

2022

  • Lechner, Severin; Malgapo, Martin Ian P.; Grätz, Christian; Steimbach, Raphael R.; Baron, Agnes; Rüther, Patrick; Nadal, Simon; Stumpf, Carmen; Loos, Christina; Ku, Xin; Prokofeva, Polina; Lautenbacher, Ludwig; Heimburg, Tino; Würf, Vivian; Meng, Chen; Wilhelm, Mathias; Sippl, Wolfgang; Kleigrewe, Karin; Pauling, Josch K.; Kramer, Karl; Miller, Aubry K.; Pfaffl, Michael W.; Linder, Maurine E.; Kuster, Bernhard; Médard, Guillaume: Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target. Nature Chemical Biology, 2022 mehr…

2018

  • Lassabe, Gabriel; Kramer, Karl; Hammock, Bruce D.; González-Sapienza, Gualberto; González-Techera, Andrés: Noncompetitive Homogeneous Detection of Small Molecules Using Synthetic Nanopeptamer-Based Luminescent Oxygen Channeling. Analytical Chemistry 90 (10), 2018, 6187-6192 mehr…

2017

  • Frejno, Martin; Zenezini Chiozzi, Riccardo; Wilhelm, Mathias; Koch, Heiner; Zheng, Runsheng; Klaeger, Susan; Ruprecht, Benjamin; Meng, Chen; Kramer, Karl; Jarzab, Anna; Heinzlmeir, Stephanie; Johnstone, Elaine; Domingo, Enric; Kerr, David; Jesinghaus, Moritz; Slotta‐Huspenina, Julia; Weichert, Wilko; Knapp, Stefan; Feller, Stephan M; Kuster, Bernhard: Pharmacoproteomic characterisation of human colon and rectal cancer. Molecular Systems Biology 13 (11), 2017, 951 mehr…
  • Klaeger, Susan; Heinzlmeir, Stephanie; Wilhelm, Mathias; Polzer, Harald; Vick, Binje; Koenig, Paul-Albert; Reinecke, Maria; Ruprecht, Benjamin; Petzoldt, Svenja; Meng, Chen; Zecha, Jana; Reiter, Katrin; Qiao, Huichao; Helm, Dominic; Koch, Heiner; Schoof, Melanie; Canevari, Giulia; Casale, Elena; Depaolini, Stefania Re; Feuchtinger, Annette; Wu, Zhixiang; Schmidt, Tobias; Rueckert, Lars; Becker, Wilhelm; Huenges, Jan; Garz, Anne-Kathrin; Gohlke, Bjoern-Oliver; Zolg, Daniel Paul; Kayser, Gian; Vooder, Tonu; Preissner, Robert; Hahne, Hannes; Tõnisson, Neeme; Kramer, Karl; Götze, Katharina; Bassermann, Florian; Schlegl, Judith; Ehrlich, Hans-Christian; Aiche, Stephan; Walch, Axel; Greif, Philipp A.; Schneider, Sabine; Felder, Eduard Rudolf; Ruland, Juergen; Médard, Guillaume; Jeremias, Irmela; Spiekermann, Karsten; Kuster, Bernhard: The target landscape of clinical kinase drugs. Science 358 (6367), 2017, eaan4368 mehr…
  • Lohse, Jonas; Schindl, Alexandra; Danda, Natasha; Williams, Chris P.; Kramer, Karl; Kuster, Bernhard; Witte, Martin D.; Médard, Guillaume: Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy. Chem. Commun. 53 (87), 2017, 11929-11932 mehr…
  • Zolg, Daniel P; Wilhelm, Mathias; Schnatbaum, Karsten; Zerweck, Johannes; Knaute, Tobias; Delanghe, Bernard; Bailey, Derek J; Gessulat, Siegfried; Ehrlich, Hans-Christian; Weininger, Maximilian; Yu, Peng; Schlegl, Judith; Kramer, Karl; Schmidt, Tobias; Kusebauch, Ulrike; Deutsch, Eric W; Aebersold, Ruedi; Moritz, Robert L; Wenschuh, Holger; Moehring, Thomas; Aiche, Stephan; Huhmer, Andreas; Reimer, Ulf; Kuster, Bernhard: Building ProteomeTools based on a complete synthetic human proteome. Nature Methods 14 (3), 2017, 259-262 mehr…

2015

  • Koch, Heiner; Busto, M. Estela Del Castillo; Kramer, Karl; Médard, Guillaume; Kuster, Bernhard: Chemical Proteomics Uncovers EPHA2 as a Mechanism of Acquired Resistance to Small Molecule EGFR Kinase Inhibition. Journal of Proteome Research 14 (6), 2015, 2617-2625 mehr…
  • Médard, Guillaume; Pachl, Fiona; Ruprecht, Benjamin; Klaeger, Susan; Heinzlmeir, Stephanie; Helm, Dominic; Qiao, Huichao; Ku, Xin; Wilhelm, Mathias; Kuehne, Thomas; Wu, Zhixiang; Dittmann, Antje; Hopf, Carsten; Kramer, Karl; Kuster, Bernhard: Optimized Chemical Proteomics Assay for Kinase Inhibitor Profiling. Journal of Proteome Research 14 (3), 2015, 1574-1586 mehr…

2011

  • Kramer, K.: Bioassays for estrogenic and androgenic effects of water constituents. In: Treatise on Water Science. Vol. 3. , 2011, 191-219 mehr…
  • Lemeer, S. ; Bluwstein, A. ; Wu, Z. ; Leberfinger, J. ; Müller, K. ; Kramer, K. ; Kuster, B.: Phosphotyrosine mediated protein interactions of the discoidin domain receptor 1. J Proteomics., 2011 mehr…
  • Wu, Z. ; Doondeea, JB. ; Moghaddas, Gholami A. ; Janning, MC. ; Lemeer, S. ; Kramer, K. ; Eccles, SA. ; Gollin, SM. ; Grenman, R. ; Walch, A. ; Feller, SM. ; Kuster, B.: Quantitative chemical proteomics reveals new potential drug targets in head and neck cancer. Mol Cell Proteomics., 2011 mehr…

2010

  • Schüller, C. ; Wiebe, JC. ; Pegel, A. ; Kramer, K. ; Skerra, A. ; Hock, B.: A system for repertoire cloning and phage display of murine and leporid antibody fragments. J AOAC Int. 93 (1), 2010, 66-79 mehr…
  • Wiebe, JC. ; Schüller, C. ; Reiche, JA. ; Kramer, K. ; Skerra, A. ; Hock, B.: An expression system for the E. coli fermentation of recombinant antibody Fab fragments from mice and rabbits. J AOAC Int. 93 (1), 2010, 80-8 mehr…

2009

  • Mahlknecht, G.; Hock, B.; Kramer, K.: Development of a Monoclonal Antibody for the Detection of a Potentially Allergenic Epitope in Soy. Analytical Letters 43 (1), 2009, 119-132 mehr…

2008

  • Kramer, K. ; Mahlknecht, G. ; Hock B: Molecular Antibody Technologies for Biosensors and Bioanalytics. In: Handbook of Biosensors and Biochips. John Wiley & Sons, Ltd, 2008 mehr…

2007

  • Kramer, K. ; Geue, H. ; Hock, B.: Directed evolution of ligand-binding proteins. In: Immunoassay and Other Bioanalytical Techniques. , 2007, 459674 mehr…
  • Kramer, K. ; Hock, B.: Recombinant Antibodies for Agrochemicals. In: Evolutionary Optimization Rational Environmental Management of Agrochemicals. , 2007, 155-170 mehr…
  • Kramer, K.; Hubauer, A.; Lausterer, R.; Salvador, J.‐P.; Marco, M.‐P.: Production of Antibodies for the Quantitative Detection of the Anabolically Active Androgens 17β‐Boldenone and Methylboldenone. Analytical Letters 40 (7), 2007, 1461-1472 mehr…

2006

  • Kaware, M. ; Bronshtein, A. ; Safi, J. ; Van, Emon JM. ; Chuang, JC. ; Hock, B. ; Kramer, K. ; Altstein, M.: Enzyme-linked immunosorbent assay (ELISA) and sol-gel-based immunoaffinity purification (IAP) of the pyrethroid bioallethrin in food and environmental samples. J Agric Food Chem. 54 (18), 2006, 6482-92 mehr…

2005

  • Cardozo, S. ; González-Techera, A. ; Last, JA. ; Hammock, BD. ; Kramer, K. ; González-Sapienza, GG.: Analyte peptidomimetics selected from phage display peptide libraries: a systematic strategy for the development of environmental immunoassays. Environ Sci Technol. 39 (11), 2005, 4234-41 mehr…
  • Kramer, K. ; Hock, B.: Molecular basis of toxic effects: Inhibition of cellular pathways and structural components. In: Plant toxicology. , 2005, 331-468 mehr…

2004

  • Kramer, K. ; Hock, B.: Antibodies for biosensors. In: Ultrathin Electrochemical Chemo- and Biosensors. , 2004, 3 - 22 mehr…

2003

  • Kramer, K. ; Hock, B.: Recombinant antibodies for environmental analysis. Anal Bioanal Chem. 377 (3), 2003, 417-26 mehr…
  • Kramer, K. ; Rau, D. ; Hock, B.: Comparison of affinity ranking and immunochemical key data as measure for molecular antibody evolution. Spectroscopy 17 (2-3), 2003, 355-65 mehr…

2002

  • Hock, B. ; Seifert, M. ; Kramer, K.: Engineering receptors and antibodies for biosensors. Biosens Bioelectron. 17 (3), 2002, 239-49 mehr…
  • Kramer, K.: Synthesis of a group-selective antibody library against haptens. J Immunol Methods. 266 (1-2), 2002, 209-20 mehr…
  • Kramer, K.: Evolutionary affinity and selectivity optimization of a pesticide-selective antibody utlizing a hapten-selective immunoglobulin repertoire. Environ Sci Technol. 36 (22), 2002, 4892-8 mehr…
  • Kramer, K. ; Fiedler, M. ; Skerra, A. ; Hock, B.: A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of F(ab) fragments and leads to improved recombinant immunoglobulin stability. Biosens Bioelectron. 17 (4), 2002, 305-13 mehr…
  • Loessner, MJ. ; Kramer, K. ; Ebel, F. ; Scherer, S.: C-terminal domains of Listeria monocytogenes bacteriophage murein hydrolases determine specific recognition and high-affinity binding to bacterial cell wall carbohydrates. Mol Microbiol. 44 (2), 2002, 335-49 mehr…
  • Maqbool, U. ; Anwar-ul-Haq. ; Qureshi, MJ. ; Iqbal, MZ. ; Hock, B. ; Kramer, K.: Development of ELISA technique for the analysis of atrazine residues in water. J Environ Sci Health B. 37 (4), 2002, 307-22 mehr…
  • Rau, D. ; Kramer, K. ; Hock, B.: Cloning, functional expression and kinetic characterization of pesticide-selective Fab fragment variants derived by molecular evolution of variable antibody genes. Anal Bioanal Chem. 372 (2), 2002, 261-7 mehr…
  • Rau, D. ; Kramer, K. ; Hock, B.: Single-chain Fv antibody-alkaline phosphatase fusion proteins produced by one-step cloning as rapid detection tools for ELISA. J Immunoassay Immunochem. 23 (2), 2002, 129-43 mehr…

2001

  • Kramer, K. ; Lepschy, J. ; Hock, B.: Long-term monitoring of atrazine contamination in soil by ELISA. J AOAC Int. 81 (1), 2001, 150-5 mehr…

2000

  • Hock, Bertold; Kramer, Karl; Seifert, Martin: Environmental Analysis Using Antibody and Receptor Based Techniques. International Journal of Environmental Analytical Chemistry 78 (3-4), 2000, 289-303 mehr…
  • Rowan, NJ. ; Candlish, AA. ; Bubert, A. ; Anderson, JG. ; Kramer, K. ; McLauchlin, J.: Virulent rough filaments of Listeria monocytogenes from clinical and food samples secreting wild-type levels of cell-free p60 protein. J Clin Microbiol. 38 (7), 2000, 2643-8 mehr…

1999

  • Hock, B; Rahman, M; Rauchalles, S; Dankwardt, A; Seifert, M; Haindl, S; Kramer, K: Stabilisation of immunoassays and receptor assays. Journal of Molecular Catalysis B: Enzymatic 7 (1-4), 1999, 115-124 mehr…

1998

  • Kramer, K. ; Knappik, A.: Strategies for recombinant antibody library synthesis: An advanced source for immunoglobulins in environmental analysis. In: Biosensors for Environmental Diagnostics. , 1998, 161-178 mehr…
  • Kramer, Karl: Synthesis of Pesticide-Specific Single-Chain FVUtilizing the Recombinant Phage Antibody System (RPAS, Pharmacia). A Detailed Protocol. Analytical Letters 31 (1), 1998, 67-92 mehr…

1997

  • Dankwardt, A.; Kramer, K.; Simon, R.; Freitag, D.; Kettrup, A.; Hock, B.: Nonextractable Pesticide Residues in Humic Substances: Immunochemical Analysis. In: ACS Symposium Series. American Chemical Society, 1997 mehr…
  • Piehler, Jacob; Brecht, Andreas; Giersch, Thomas; Kramer, Karl; Hock, Bertold; Gauglitz, Günter: Affinity characterization of monoclonal and recombinant antibodies for multianalyte detection with an optical transducer. Sensors and Actuators B: Chemical 39 (1-3), 1997, 432-437 mehr…

1996

  • Kramer, K. ; Hock, B.: Recombinant antibodies for pesticide immunoanalysis. In: Immunoassays for Residue Analysis. , 1996, 471-484 mehr…
  • kramer, karl; Hock, Bertold: Recombinant single‐chain antibodies againsts‐Triazines. Food and Agricultural Immunology 8 (2), 1996, 97-109 mehr…

1995

  • Dankwardt, Andrea; Pullen, Sabine; Rauchalles, Stefanie; Kramer, Karl; Just, Franziska; Hock, Bertold; Hofmann, Ralf; Schewes, Robert; Maidl, Franz Xaver: Atrazine Residues in Soil Two Years After the Atrazine Ban - A Comparison of Enzyme Immunoassay with HPLC. Analytical Letters 28 (4), 1995, 621-634 mehr…
  • Giersch, T. ; Kramer, K.: Production and application of monoclonal antibodies for the determination of pesticides. In: Immunochemical Detection of Pesticides and Their Metabolites in the Water Cycle. , 1995, 25 - 42 mehr…
  • Hock, B. ; Giersch, T. ; Kramer, K. ; Dankwardt, A.: Antibody production and progress in hybridoma technology by immunomagnetic screening. In: New Frontiers in Agrochemical Immunoassay. , 1995, 149-162 mehr…
  • Hock, Bertold; Dankwardt, Andrea; Kramer, Karl; Marx, Alexander: Immunochemical techniques: Antibody production for pesticide analysis. A review. Analytica Chimica Acta 311 (3), 1995, 393-405 mehr…
  • Piehler, J. ; Brecht, A. ; Kramer, K. ; Hock, B. ; Gauglitz, G.: Multi-analyte determination with a direct optical multi-antibody detection system. In: Environmental Monitoring and Hazardous Waste Site Remediation. , 1995, 185-193 mehr…

1994

  • Hock, Bertold; Giersch, Thomas; Dankwardt, Andrea; Kramer, Karl; Pullen, Sabine: Toxicity assessment and on-line monitoring: Immunoassays. Environmental Toxicology & Water Quality 9 (4), 1994, 243-262 mehr…
  • Kramer, Karl; Giersch, Thomas; Hock, Bertold: Magnetic bead selection of hybridomas producing pesticide antibodies. Food and Agricultural Immunology 6 (1), 1994, 5-16 mehr…

1993

  • Giersch, T. ; Kramer, K. ; Hock, B.: Optimization of a monoclonal antibody-based enzyme immunoassay for the detection of terbuthylazine. Sci Total Environ. 132 (2-3), 1993, 435-48 mehr…
  • Giersch, T. ; Kramer, K. ; Weller, M. ; Hock, B.: Improvement of a monoclonal antibody-based immunoassay for the determination of terbutryn. Acta hydrochim hydrobiol 21 (6), 1993, 312-5 mehr…
  • Hock, Bertold; Giersch, Thomas; Kramer, Karl-Josef: Monoclonal antibody-based enzyme immunoassays for the sensitive detection of s-triazines in water. International Conference on Monitoring of Toxic Chemicals and Biomarkers, SPIE, 1993 mehr…