OPTIMIZATION OF THE KINOBEADS TECHNOLOGY FOR DRUG PROFILING
|Project Members:||Guillaume Médard, Benjamin Ruprecht, Fiona Pachl, Susan Kläger|
In conjuction with quantitative mass spectrometry, the kinobeads technology has proven to be an extremely useful tool for the unbiased profiling of kinase inhibitors. These profiles are only as good as the native kinome the kinobeads allow to investigate. Hence a careful selection and combination of chemical probes and cell lysates would allow to generate an optimal affinity matrix and a human (or murine) native kinase repertoire that would enhance the kinome coverage of the assay. The aim of the project is twofold. 1) To characterize the kinomes of many cell lines systematically to generate a freely available database showing the differential abundance of kinases in these cell lines. 2) To synthesize and characterize the most promiscuous affinity probes to generate another freely available database allowing to predict the best combinations of complementary probes to cover specific sub-kinomes.