Fractionation strategies on peptide level

Recently we developed a trimodal mixed phase column chromatography incorporating polar embedded reversed phase, weak anion exchange and strong cation exchange material. This highly MS compatible fractionation strategy is complemented by hydrophilic strong anion exchange column chromatography and tip-based basic reversed phase fractionation methods to facilitate a deeper proteome and PTM profiling with higher sequence coverage enabling the identification and quantification of >10,000 proteins and modification events.