Automated solid-phase peptide synthesis (SPPS)

Producing large numbers of pure peptides in an unattended fashion and in a reasonable amount of time is a feature of automated SPPS. Apart from speed and throughput, SPPS has become quite versatile as many different reagents offering special protecting groups and specifically designed solid supports have become available. For our needs, we mostly employ the FMOC strategy (PyBOP coupling method) on an INTAVIS MultiPep SPOT to synthesise plain, phosphorylated and glycosylated peptides. The versatility of this instrument also allows us to generate high density peptide arrays on cellulose membranes.